Isolement et caractérisation chimique des lipopolysaccharides de type R dans une souche hypovirulente de Yersinia pestis
| Autor: | M Bruneteau, S Minka |
|---|---|
| Rok vydání: | 1998 |
| Předmět: |
Arabinose
Gel electrophoresis biology Lipopolysaccharide Chemistry Immunology Mineralogy Biological activity General Medicine biology.organism_classification Applied Microbiology and Biotechnology Microbiology Enterobacteriaceae Molecular biology Lipid A chemistry.chemical_compound Yersinia pestis Genetics lipids (amino acids peptides and proteins) Molecular Biology Polyacrylamide gel electrophoresis |
| Zdroj: | Canadian Journal of Microbiology. 44:477-481 |
| ISSN: | 1480-3275 0008-4166 |
| Popis: | The lipopolysaccharides LPS I and LPS II, isolated from the hypovirulent EV40 strain of Yersinia pestis, are composed only of type R lipopolysaccharides. This type consists of two forms, a and b, depending on their solubility pattern in a solvent mixture containing varying proportions of chloroform, methanol, hexane, and hydrochloric acid. LPS I consists of one subtype, RIb, while LPS II consists of two subtypes, RIIa and RIIb. Analysis by gel electrophoresis shows that the mass of these lipopolysaccharide forms are in the vicinity of 2000-3000 Da. The RIb and RIIb subtypes, which are found in the majority of lipopolysaccharide I and II fractions, are composed of ketoses and amines that are similar to those occurring in LPS I and LPS II. In contrast, the two subtypes RIIa and RIIb are different both in terms of the composition of lipid A and the extent of its substitution. Certain fractions of RIIa contain only lipid A and 3-deoxy-D-manno-octulosonic acid (KDO), while other fractions of RIIb possess a lipid A, which is not substituted by arabinose. The whole set of these R-type lipopolysaccharide forms are excellent models for the study of the role of the primary structure of the polysaccharide region, and for the effect of lipid A substitution on the biological activity of bacterial lipopolysaccharides.Key words: Yersinia pestis, hypovirulence, lipopolysaccharides, R type.[Journal translation] |
| Databáze: | OpenAIRE |
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