PCR Technique Assisting the Early Diagnosis of Human Papillomavirus A retrospective clinical study
| Autor: | Aurel Claudiu Vartolomei, Adrienn Dobai, Daniela Manuc, Zoltan Magyar, Csaba Dobo Nagy, Karoly Mensch, Henrik Mensch, Mariana Pacurar, Krisztina Szarka |
|---|---|
| Rok vydání: | 2018 |
| Předmět: |
medicine.medical_specialty
Process equipment Materials Science (miscellaneous) Process Chemistry and Technology General Engineering virus diseases General Chemistry General Medicine 010402 general chemistry 01 natural sciences female genital diseases and pregnancy complications General Biochemistry Genetics and Molecular Biology 0104 chemical sciences Retrospective data Materials Chemistry medicine Medical physics General Pharmacology Toxicology and Pharmaceutics Human papillomavirus |
| Zdroj: | Revista de Chimie. 69:2781-2787 |
| ISSN: | 2668-8212 0034-7752 |
| DOI: | 10.37358/rc.18.10.6624 |
| Popis: | The number of oral cancers associated with Human Papillomavirus (HPV) infection is increasing worldwide. The purpose of this retrospective clinical trial is to investigate the relationship between genital and oral HPV infection and to observe the possibilities of polymerase chain reaction (PCR) technique and the limitations for oral HPV infection. The results of genital and oral HPV screening of 34 female patients and their partner, and 14 single female patients were processed in a private practice in Budapest between 2012 and 2015. After brush-biopsy sampling, HPV DNA identification and typing were performed using PCR technique. All in all 164 samples were typed. HPV DNA was detected in 76 cases (48.20%). HPV was detected in 55 cases from the genital samples (67%), and in 21 cases from the oral samples (25.6%). Genital HPV infection was statistically significantly higher in women (79.2% vs 22,9%, p[0.001). Gender comparison of genital HPV infection showed a statistically significant difference (79.2% for women, 50% for men, p = 0.006). In case of genital HPV infection, the oral HPV infection of the same person is more frequent. In this study, the above mentioned difference is significant for women and not significant for men. HPV16 is the HPV genotype which was identified in the highest ratio(47.2%). PCR technique is capable of the detection of oral HPV infection, but follow-up studies with higher case number , and questionnaire studies are needed to understand HPV transmission more accurately. |
| Databáze: | OpenAIRE |
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