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Background: Abnormal wound repair is implicated in asthma pathogenesis. The nose is the point of first contact with the environment, yet wound repair ability of the nasal epithelium has received little attention. We sought to determine the impact of atopy and asthma on wound healing of nasal epithelium. Methods: Primary nasal epithelial cells harvested from adult volunteers classified into mutually exclusive groups [healthy (H), atopic non-asthmatic (ANA), non-atopic asthmatic (NAA) and atopic asthmatic (AA)] were grown into well-differentiated epithelium at the air-liquid interface. The ability of the epithelium to heal a mechanical wound was determined under various conditions. Results: Wound healing rate (%/hour) was slowest in ANA (2.9±1.8, vs 4.3±1.9 in H, p=0.02). Healing rates of AA (3.8±1.0) and NAA (4.1±1.1) were not different from H. Exogenous IL-13 slowed healing (2.2±1.1 vs 4.0±1.3, p |
