Quantitative, phenotypic, and functional evaluation of basophils in myelodysplastic syndromes
| Autor: | Peter Valent, H J Bühring, Otto Götze, Y Majlesi, Klaus Lechner, Minoo Ghannadan, Joerg Zwirner, Alexander W. Hauswirth, Gerit-Holger Schernthaner, Wolfgang Füreder, Wolfgang R. Sperr, H Semper |
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| Rok vydání: | 2001 |
| Předmět: |
Clinical Biochemistry
chemical and pharmacologic phenomena CD59 Basophil Immunoglobulin E Biochemistry 03 medical and health sciences chemistry.chemical_compound 0302 clinical medicine hemic and lymphatic diseases medicine 030304 developmental biology Whole blood 0303 health sciences Cytopenia biology Myelodysplastic syndromes hemic and immune systems General Medicine medicine.disease 3. Good health medicine.anatomical_structure chemistry Immunology biology.protein Interleukin-3 receptor Histamine 030215 immunology |
| Zdroj: | European Journal of Clinical Investigation. 31:894-901 |
| ISSN: | 0014-2972 |
| DOI: | 10.1046/j.1365-2362.2001.00887.x |
| Popis: | Background The myelodysplastic syndromes (MDS) are a group of clonal haematological disorders characterized by cytopenia(s), reduced differentiation-capacity of myeloid cells, and impaired leukocyte function. However, little is known so far about basophil granulocytes in MDS. Design We have compared the numbers, phenotype and function of basophils in MDS patients with those in healthy subjects. A total numer of 23 patients with MDS (refractory anaemia, n = 8; refractory anaemia with ringsideroblasts, n = 7; refractory anaemia with excess of blasts/refractory anaemia with excess of blasts in transformation, n = 8) and 20 healthy donors were included. Results The numbers of blood basophils in MDS patients (34.6 +/- 62.9 microL-1) was lower compared to healthy controls (58.6 +/- 64.9 microL-1). Correspondingly, whole blood histamine levels were lower in MDS patients (MDS 34.1 +/- 29.1 ng mL-1 vs. normal donors 72.0 +/- 36.9 ng mL-1). Like "normal" basophils, basophils in MDS expressed interleukin-3 receptor alpha (CD123), E-NPP3 (CD203c), CR1 (CD35), CR3 (CD11b), CR4 (CD11c), membrane co-factor protein (CD46), decay-accelerating factor (CD55) and membrane attack complex inhibitory factor (CD59), as well as receptors for C3a, C5a (CD88), and IgE. Recombinant human (rh) C5a and anti-IgE induced significant release of histamine from basophils in both groups of donors without significant differences between MDS and healthy controls. Conclusions The absolute numbers of basophils in MDS patients are lower than in normal donors. However, basophils in MDS do not differ from their "normal counterparts" in terms of complement receptor expression, IgE-receptor expression, or functional responses to respective ligands. |
| Databáze: | OpenAIRE |
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