Seven days ex vivo perfusion of whole ewe ovaries with follicular maturation and oocyte retrieval: towards the development of an alternative fertility preservation method
| Autor: | Panagiotis Tsiartas, Claudia Mateoiu, Meghshree Deshmukh, Debashish Banerjee, Arvind M. Padma, Milan Milenkovic, Fulvio Gandolfi, Mats Hellström, Pasquale Patrizio, Randa Akouri |
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| Rok vydání: | 2022 |
| Předmět: | |
| Zdroj: | Reproduction, Fertility and Development. 34:331-342 |
| ISSN: | 1448-5990 1031-3613 |
| Popis: | Fertility preservation methods for prepubertal women about to undergo gonadotoxic chemo and/or radiation therapy are limited. Therefore, the aim of this study was to investigate the feasibility to develop an alternative fertility preservation method based on an ex vivo perfusion platform for whole ewe ovaries. Thirteen ewe ovaries were divided into two groups (group 1 and 2) that were perfused in a bioreactor for up to 7 days. Group 1 (n = 3) were stimulated with human menopausal gonadotropin (hMG) administered in single daily dose, while group 2 (n = 10) were stimulated continuously for 24 h. The perfused ovaries in group 1 showed no significant differences in follicular density, sub-follicular morphology and oocyte quality after ischaemia and after ex vivo perfusion compared with non-perfused control ovaries. The perfused ovaries in group 2 showed a significant decrease in the follicular reserve and oocyte quality compared with the control group. In total, 16 GV–MI oocytes were retrieved from both groups. This study describes for the first time the ex vivo maintenance of viable follicles of ewe ovaries with oocyte integrity and the retrieval of oocytes after ex vivo hormonal perfusion with two different protocols for up to 7 days. |
| Databáze: | OpenAIRE |
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