Easy-to-attach vacuum modules with biochips for droplets generation from small sample volumes
| Autor: | Chia-Hung Lee, Chien-Chong Hong |
|---|---|
| Rok vydání: | 2016 |
| Předmět: |
Range (particle radiation)
Materials science business.industry 010401 analytical chemistry Nanotechnology Small sample 02 engineering and technology Deformation (meteorology) 021001 nanoscience & nanotechnology Condensed Matter Physics Chip medicine.disease_cause 01 natural sciences 0104 chemical sciences Electronic Optical and Magnetic Materials Volumetric flow rate Shape-memory polymer Mold Materials Chemistry medicine Optoelectronics 0210 nano-technology Biochip business |
| Zdroj: | Microfluidics and Nanofluidics. 20 |
| ISSN: | 1613-4990 1613-4982 |
| DOI: | 10.1007/s10404-016-1821-3 |
| Popis: | This study developed a droplet biochip driven with a single vacuum module to produce droplets from small sample volumes. The vacuum module is composed of a shape memory polymer, which releases prestored energy for shape recovery when subjected to heat trigger, and works as an easy-to-attach vacuum source. The three-layer Teflon mold is designed to manufacture a vacuum module with a favorable yield (>95%). The water-in-oil emulsion droplets can be produced by attaching a single vacuum module with a microfluidic chip. The diameter of the vacuum module has been successfully reduced to 6 mm. The maximum driving pressure provided by the 15-mm diameter vacuum module attached with a 2 μL chip is approximately 9653 Pa. The produced flow rate varies with the deformation rate of the vacuum module and becomes stable at 2.4 µL/min during the droplet generation. The droplet diameters range from 180 to 240 µm. The developed disposable vacuum module is easy to attach, easy to use, easy to make, cost-effective, and automatically controllable for driving fluids on a chip for handling small sample volumes. |
| Databáze: | OpenAIRE |
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