Practical method combining loop-mediated isothermal amplification and bait trap to detect Pythium helicoides from hydroponic culture solutions
| Autor: | Masashi Matsusaki, Noriyuki Miyake, Ryoji Suzuki, Shinro Kato, Yasushi Ishiguro, Shiro Fukuta, Reiko Takahashi, Hirofumi Nagai |
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| Rok vydání: | 2016 |
| Předmět: |
0106 biological sciences
0301 basic medicine Hydroponic culture Loop-mediated isothermal amplification Plant Science Biology Trap (plumbing) Perilla 01 natural sciences 03 medical and health sciences Horticulture 030104 developmental biology Pythium helicoides Botany Agronomy and Crop Science Mycelium 010606 plant biology & botany |
| Zdroj: | Journal of General Plant Pathology. 83:1-6 |
| ISSN: | 1610-739X 1345-2630 |
| DOI: | 10.1007/s10327-016-0691-6 |
| Popis: | Two detection methods combining loop-mediated isothermal amplification (LAMP) and a bait trap were developed to detect Pythium helicoides in greenhouses containing roses, miniature roses, and poinsettias in hydroponic culture systems. In “Bait-LAMP”, a crude extract derived from perilla seeds as the bait was used in the LAMP reaction, whereas in the “Bait culture-LAMP”, a crude extract of mycelia grown out from perilla seeds onto Pythium-selective medium served as the bait. The two methods are simple and rapid for practical monitoring of P. helicoides in hydroponic culture systems. |
| Databáze: | OpenAIRE |
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