β-NaGdF4:Eu3+ nanocrystal markers for melanoma tumor imaging
| Autor: | Alexander Lutsyk, Bartlomiej Sojka, Jan Misiewicz, Mateusz Banski, Tetiana Dumych, Rostyslav Bilyy, S. Drobczynski, A. Podhorodecki, M. M. Lutsyk |
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| Rok vydání: | 2016 |
| Předmět: |
Fluorescence-lifetime imaging microscopy
Chemistry General Chemical Engineering Melanoma Nanotechnology 02 engineering and technology General Chemistry Conjugated system 010402 general chemistry 021001 nanoscience & nanotechnology medicine.disease 01 natural sciences 0104 chemical sciences Nanocrystal Covalent bond medicine Fluorescence microscope Biophysics 0210 nano-technology Ex vivo Nanoconjugates |
| Zdroj: | RSC Advances. 6:57854-57862 |
| ISSN: | 2046-2069 |
| DOI: | 10.1039/c6ra10351k |
| Popis: | In the current paper, the strategy of tuning the optical and structural properties of Eu doped nanocrystals (NCs) is described with the emphasis on obtaining NCs with high brightness and photostability at the desired excitation and emission range. Those NCs are further chemically functionalized and covalently conjugated with lectins, providing specific (but reversible) targeting of pathological glycans on the surface of melanoma cells. Nanoconjugates are then injected intravenously into the blood stream of laboratory mice. Subsequently, histological slices were examined by fluorescence microscopy, whereas whole melanoma tumor were examined by ex vivo fluorescence imaging. Analysis revealed specific targeting, high intensity and photostability of the signal during visualization. |
| Databáze: | OpenAIRE |
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