Technical Notes: Blood Preparation for Flow Cytometry to Identify Triploidy in Grass Carp

Autor:	Lars W. J. Anderson, Robert T. Pine
Rok vydání:	1990
Předmět:	Genetics Veterinary medicine medicine.diagnostic_test biology Coulter counter medicine Aquatic animal General Agricultural and Biological Sciences biology.organism_classification Two Hundred Fifty Flow cytometry Grass carp Aquatic organisms
Zdroj:	The Progressive Fish-Culturist. 52:266-268
ISSN:	1548-8640 0033-0779
DOI:	10.1577/1548-8640(1990)052<0266:tnbpff>2.3.co;2
Popis:	Blood from grass carp (Ctenopharyngodon idella), ranging in size between 50 and 450 g, was prepared for flow cytometry with fewer steps than have previously been recorded. Two hundred fifty ploidy determinations have been made in 2 h by a two-person team with much less effort than had previously been the norm. This technique compares favorably in efficiency to and is reportedly more accurate than the Coulter counter because of the direct measurement of blood DNA content.
Databáze:	OpenAIRE
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