ANALYSIS OF MALTODEXTRINS IN PLANT EXTRACTS BY LC USING EVAPORATIVE LIGHT SCATTERING DETECTION

Autor:	J. M. Seigneuret, Michel Dreux, S. Guenu
Rok vydání:	2000
Předmět:	Chromatography Chemistry Elution Clinical Biochemistry Analytical chemistry Pharmaceutical Science Maltodextrin Biochemistry High-performance liquid chromatography Light scattering Analytical Chemistry chemistry.chemical_compound Column chromatography Chromatography detector Desorption Phase (matter)
Zdroj:	Journal of Liquid Chromatography & Related Technologies. 23:2883-2896
ISSN:	1520-572X 1082-6076
DOI:	10.1081/jlc-100101240
Popis:	Two liquid chromatography methods using an evaporative light scattering detection (ELSD) have been developed to determine the carbohydrate profile of maltodextrins. The first method requires an octadecyl-bonded silica column and a methanol/water mobile phase. With the second method, an amino-bonded polymeric stationary phase and an acetonitrile/water eluent are used. In the two cases, a gradient elution was necessary to desorb the higher molecular weight polysaccharides. This elution mode was perfectly compatible with ELSD since no baseline drift was observed. These two methods were applied to quantify maltodextrins present in plant spray dried-powder of melilot and basil.
Databáze:	OpenAIRE
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