Microbiome-dependent modulation of mucosal immunity at the ocular surface
| Autor: | Anthony J St. Leger, Jigar V Desai, Rebecca Drummond, Abirami Kugadas, Fatimah Almaghrabi, Phyllis Silver, Mihaela Gadjeva, Yoichiro Iwakura, Michail S Lionakis, Rachel R Caspi |
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| Rok vydání: | 2017 |
| Předmět: | |
| Zdroj: | The Journal of Immunology. 198:149.6-149.6 |
| ISSN: | 1550-6606 0022-1767 |
| DOI: | 10.4049/jimmunol.198.supp.149.6 |
| Popis: | Mucosal sites such as the intestine, oral cavity, nasopharynx, and female reproductive tract all have their associated commensal flora. The surface of the eye (conjunctiva) is also a mucosal site, but existence of a resident microbiome on the ocular surface is highly controversial. We used a mouse model of ocular surface disease to study whether commensal microbes are present in ocular mucosa and modulate immunity. We found that IL-17 is constitutively produced within the conjunctiva-associated lymphoid tissue (CALT) and recruits neutrophils to the ocular surface in the steady state and after a bacterial challenge. IL-17 sources in CALT include γδ T cells, αβ T cells and innate lymphoid cells (ILCs), in that order. Notably, a strain of Corynebacterium isolated from ocular tissue of mice, and known to occur on the ocular surface of humans, induced the conjunctival γδ T cells to secrete IL-17, which in turn modified the local inflammatory signature and barrier function. Elimination of this putative commensal by antibiotic treatment, or its introduction into non-colonized mice, correlated inversely with severity of an experimental Candida albicans infection. Our results thus indicate that a relationship exists between commensals and immune cells at the ocular surface, which is critical for maintenance of homeostasis and host defense within the ocular mucosa. |
| Databáze: | OpenAIRE |
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