An immobilized and highly stabilized self-sufficient monooxygenase as biocatalyst for oxidative biotransformations
| Autor: | Marina Guillén, Gregorio Álvaro, Josep López-Santín, Maximilian J. L. J. Fürst, Daniela Valencia |
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| Rok vydání: | 2017 |
| Předmět: |
010405 organic chemistry
Renewable Energy Sustainability and the Environment Chemistry Iminodiacetic acid General Chemical Engineering Organic Chemistry Cyclohexanone Substrate (chemistry) Monooxygenase 010402 general chemistry 01 natural sciences Pollution 12. Responsible consumption 0104 chemical sciences Baeyer–Villiger oxidation Inorganic Chemistry chemistry.chemical_compound Fuel Technology Biocatalysis Atom economy Organic chemistry Bifunctional Waste Management and Disposal Biotechnology |
| Zdroj: | Journal of Chemical Technology & Biotechnology. 93:985-993 |
| ISSN: | 0268-2575 |
| Popis: | BACKGROUND The requirement of expensive cofactors that must be efficiently recycled is one of the major factors hindering the wide implementation of industrial biocatalytic oxidation processes. In this research, a sustainable approach based on immobilized self-sufficient Baeyer-Villiger monooxygenases is discussed. RESULTS A bifunctional biocatalyst composed of an NADPH-dependent cyclohexanone monooxygenase (CHMO) fused to an NADP+-accepting phosphite dehydrogenase (PTDH) catalyzes e-caprolactone synthesis from cyclohexanone, using phosphite as a cheap sacrificial substrate for cofactor regeneration. Several immobilized derivatives of the fused enzyme have been prepared with high immobilization yield (97%); the one obtained by affinity adsorption on Co-IDA (Co: cobalt chelated; IDA: iminodiacetic acid) support has shown to be highly stable affording average yields of 80 % after 18 reaction cycles. CONCLUSIONS The immobilized self-sufficient monooxygenase has demonstrated to be able to perform Baeyer Villiger oxidation with efficient cofactor recovery and biocatalyst recycling. The proposed biocatalytic process offers access to valuable molecules with high atom economy and limited waste generation. |
| Databáze: | OpenAIRE |
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