Modulation of phosphatidylinositol 3-kinase activity duringin vitrooocyte maturation increases the production of bovine blastocysts
| Autor: | Roger Cardoso Maia, Alinne Glória Curcio, Diego Fernando Dubeibe Marín, Edgar Mauricio Mogollón-Waltero, K.S. Viana, Maria Clara Caldas Bussiere, Janaina Leite Pereira, Laura Mathias Barroso, Helga Fernandes Gomes, Angelo José Burla Dias |
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| Rok vydání: | 2020 |
| Předmět: | |
| Zdroj: | Zygote. 28:371-376 |
| ISSN: | 1469-8730 0967-1994 |
| Popis: | SummaryThis study aimed to evaluate the effect of regulating phosphatidylinositol 3-kinase (PI3K) activity on the kinetics of oocyte nuclear maturation and the blastocyst rate. To evaluate oocyte viability, nuclear maturation rate andin vitroembryo production, cumulus–oocyte complexes (COCs) were maintained for 0, 10 min, 6 h or 22 h in TCM 199 medium supplemented with 20 nM wortmannin, an inhibitor of PI3K. After each period, COCs were transferred to the same medium without wortmannin and kept under the same conditions until completion of 22 h ofin vitromaturation (IVM). To evaluate the effect of time on progression of nuclear maturation, COCs cultivated with 20 nM wortmannin was maintained for 22, 28 or 34 h of IVM. To determine the effect of wortmannin on the activity of maturation-promoting factor (MPF), COCs were kept under IVM conditions in the presence of the inhibitor for 0, 1, 3, 6, or 8 h. Exposure of COCs to wortmannin decreased (P< 0.05) the percentage of oocytes that reached metaphase II (MII) up to 22 h, MPF activity and reduced PI3K activity by 30%. However, after 28 and 34 h, 70% of oocytes reached the MII stage in the presence of inhibitor Moreover, COCs matured in the presence of wortmannin showed an increase (P< 0.05) in the blastocyst rate. These findings suggested that the regulation of the PI3K activity during IVM of bovine COCs interfered with the meiotic progression due to control of MPF activity, positively affecting the blastocyst rate. |
| Databáze: | OpenAIRE |
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