Application of Prohibitin Immunohistochemistry to Rat Livers Treated with Methapyrilene Hydrochloride

Autor:	Mami Kouchi, Takatoshi Koujitani, Takaki Seki, Izumi Matsumoto, Shinichi Mikami, Kaoru Toyosawa, Tadashi Inoue, Yoshiko Michimae
Rok vydání:	2008
Předmět:	Pathology medicine.medical_specialty Biology Mitochondrion Toxicology Pathology and Forensic Medicine law.invention Muscle hypertrophy law Cytoplasm Ultrastructure medicine biology.protein Immunohistochemistry Electron microscope Antibody Prohibitin
Zdroj:	Journal of Toxicologic Pathology. 21:119-122
ISSN:	1881-915X 0914-9198
Popis:	An immunohistochemical staining method for prohibitin, a 30-kDa protein located in the inner membrane of mitochondria, is used for detection of mitochondria on formalin-fixed paraffin-embedded sections. Methapyrilene hydrochloride (MP), which is known to increase mitochondria in periportal hepatocytes, was administered once by oral gavage to male rats at a dose of 100 mg/kg. Hypertrophy with eosinophilic granules was noted in the periportal hepatocytes treated with MP. Immunohistochemical staining with prohibitin antibody demonstrated a positive reaction to eosinophilic granules. Electron microscopy showed an increased number of mitochondria in the periportal hepatocytes treated with MP compared to the control. Although electron microscopy is a useful tool to detect ultrastructural changes, an immunohistochemical examination with anti-prohibitin antibody is a simple and easy method for detecting cytoplasmic mitochondria quantitatively.
Databáze:	OpenAIRE
Externí odkaz:	https://explore.openaire.eu/search/publication?articleId=doi_________::e37cc882c647db8b75ebda2d513d62ff https://doi.org/10.1293/tox.21.119 Zobrazit plný text záznamu