Comparison of Impregnated Bone Morphogenetic Protein-2 Release Kinetics from Biopolymer Scaffolds
| Autor: | E. V. Galitsyna, Vladimir K. Popov, T. B. Bukharova, Igor I. Babichenko, E. O. Osidak, A. V. Vasilyev, V. S. Kuznetsova, E. N. Antonov, Timofei E. Grigoriev, Sergey N. Chvalun, Sergey Petrovich Domogatsky, A. A. Kulakov, D. V. Goldshtein, S. A. Minaeva, Yu. D. Zagoskin |
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| Rok vydání: | 2019 |
| Předmět: |
010302 applied physics
Materials science Biocompatibility Kinetics General Engineering 02 engineering and technology engineering.material 021001 nanoscience & nanotechnology 01 natural sciences Bone morphogenetic protein 2 Supercritical fluid Chitosan chemistry.chemical_compound chemistry 0103 physical sciences Highly porous engineering Biophysics General Materials Science Biopolymer 0210 nano-technology Cytotoxicity |
| Zdroj: | Inorganic Materials: Applied Research. 10:1093-1100 |
| ISSN: | 2075-115X 2075-1133 |
| Popis: | The purpose of this study was to evaluate the release kinetics of impregnated recombinant human bone morphogenetic protein-2 (rhBMP-2) from different engineered scaffolds. Poly(lactide-co-glycolide) (PLG) matrices prepared by supercritical fluid technologies (SCFT) showed the highest biocompatibility and long-term release of rhBMP-2. There was an even release of rhBMP-2 from them for 11 days. The subsequent use of laser sintering allowed delaying the peak of the protein release for a period of 13 to 15 days. The average loss of rhBMP-2 using SCFT did not exceed 20%. The maximum release of rhBMP-2 from a collagen-fibronectin hydrogel was in the period from 4 to 6 days. But 47 ± 12% rhBMP-2 loss was shown. Highly porous polylactide-based scaffolds obtained by freeze-drying were inferior to other scaffolds in their ability to release rhBMP-2 for a prolonged period. The hydrogel and chitosan-based granules showed high cytotoxicity and a short period of protein release. |
| Databáze: | OpenAIRE |
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