| Autor: |
Allan S. Hoffman, Wayne R. Gombotz, Satoshi Uenoyama, Gottfried Schmer |
| Rok vydání: |
1985 |
| Předmět: |
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| Zdroj: |
Radiation Physics and Chemistry (1977). 25:549-556 |
| ISSN: |
0146-5724 |
| DOI: |
10.1016/0146-5724(85)90134-7 |
| Popis: |
The enzyme asparaginase was immobilized onto a porous hollow polypropylene (PP) fiber blood plasma exchange device for the treatment of acute lymphocytic leukemia. The devices were first radiation grafted with polymethacrylic acid (poly(MAAc)). This introduces carboxyl groups onto the surface of the fibers. Several variables were studied in the grafting reaction including the effects of solvent type and monomer concentration. The carboxyl groups were activated with N-hydroxy succinimide (NHS) using carbodiimide chemistry. Asparaginase was then covalently immobilized on the activated surfaces. Quantitative relationships were found relating the percent graft to the amount of immobilized enzyme which was active. The enzyme reactor was tested both in vitro and in vivo using a sheep as an animal model. |
| Databáze: |
OpenAIRE |
| Externí odkaz: |
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