Dynamics of amino acid redistribution in the carnivorous Venus flytrap (Dionaea muscipula) after digestion of13C/15N-labelled prey
| Autor: | Heinz Rennenberg, Jörg Kruse, Peng Gao, Monika Eibelmeier, Saleh A. Al-Farraj |
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| Rok vydání: | 2017 |
| Předmět: |
0106 biological sciences
0301 basic medicine chemistry.chemical_classification biology media_common.quotation_subject Heterotroph chemistry.chemical_element Plant Science General Medicine Insect biology.organism_classification Photosynthesis 01 natural sciences Nitrogen Predation Amino acid 03 medical and health sciences 030104 developmental biology chemistry Botany Venus flytrap Droseraceae Ecology Evolution Behavior and Systematics 010606 plant biology & botany media_common |
| Zdroj: | Plant Biology. 19:886-895 |
| ISSN: | 1435-8603 |
| Popis: | Amino acids represent an important component in the diet of the Venus flytrap (Dionaea muscipula), and supply plants with much needed nitrogen resources upon capture of insect prey. Little is known about the significance of prey-derived carbon backbones of amino acids for the success of Dionaea's carnivorous life-style. The present study aimed at characterizing the metabolic fate of 15 N and 13 C in amino acids acquired from double-labeled insect powder. We tracked changes in plant amino acid pools and their δ13 C- and δ15 N-signatures over a period of five weeks after feeding, as affected by contrasting feeding intensity and tissue type (i.e., fed and non-fed traps and attached petioles of Dionaea). Isotope signatures (i.e., δ13 C and δ15 N) of plant amino acid pools were strongly correlated, explaining 60% of observed variation. Residual variation was related to contrasting effects of tissue type, feeding intensity and elapsed time since feeding. Synthesis of nitrogen-rich transport compounds (i.e., amides) during peak time of prey digestion increased 15 N- relative to 13 C- abundances in amino acid pools. After completion of prey digestion, 13 C in amino acid pools was progressively exchanged for newly fixed 12 C. The latter process was most evident for non-fed traps and attached petioles of plants that had received ample insect powder. We argue that prey-derived amino acids contribute to respiratory energy gain and loss of 13 CO2 during conversion into transport compounds (i.e., 2 days after feeding), and that amino-nitrogen helps boost photosynthetic carbon gain later on (i.e., 5 weeks after feeding). |
| Databáze: | OpenAIRE |
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