Sensor Array: Impedimetric Label-Free Sensing of DNA Hybridization in Real Time for Rapid, PCR-Based Detection of Microorganisms

Autor:	Maria W. Smith, David R. Evans, Andrei L. Ghindilis, Kevin R. Schwarzkopf, António M. Baptista, Holly M. Simon, Changqing Zhan
Rok vydání:	2009
Předmět:	Detection limit Materials science Microbial DNA DNA–DNA hybridization Nanotechnology Signal Analytical Chemistry law.invention Sensor array law Electrochemistry Oligomer restriction Biological system Biosensor Polymerase chain reaction
Zdroj:	Electroanalysis. 21:1459-1468
ISSN:	1521-4109 1040-0397
Popis:	A novel, impedance-based electronic sensor format was used for label-free, real-time detection of microbial DNA on oligonucleotide probe arrays. Detection limits of 5–10 nM were achieved for single-stranded, PCR-amplified DNA targets. Hybridization selectivity yielded 9- to 12-fold signal increases for specific targets, and the sensor arrays were re-used multiple times without significant signal degradation. These and other features of the SHARP Laboratories of America (SLA) sensor array, such as its ability to acquire continuous measurements of DNA as it accumulates on the array surface, make it an attractive biosensor platform for field detection and monitoring of sentinel and/or pathogenic microorganisms.
Databáze:	OpenAIRE
Externí odkaz:	https://explore.openaire.eu/search/publication?articleId=doi_________::e365fa225479c13ce121c0a8ed8fa05f https://doi.org/10.1002/elan.200904549 Zobrazit plný text záznamu Plný text