Centromere sequences localize to the nuclear halo of human spermatozoa
| Autor: | Yuval Yaron, K. Gyi, Saleh A.D. Ebrahim, M. P. Johnson, S. A. Krawetz, M. I. Evans, J. A. Kramer |
|---|---|
| Rok vydání: | 1998 |
| Předmět: |
medicine.diagnostic_test
Urology Endocrinology Diabetes and Metabolism Halo nucleus Biology Nuclear matrix Genome Molecular biology Cell biology Chromatin Cell nucleus medicine.anatomical_structure Reproductive Medicine Centromere medicine Scaffold/matrix attachment region Fluorescence in situ hybridization |
| Zdroj: | International Journal of Andrology. 21:13-18 |
| ISSN: | 0105-6263 |
| Popis: | Chromatin is organized into a series of discrete nuclear matrix-associated and non-nuclear matrix-associated domains. The non-matrix-associated domains consist of loops of DNA that are attached to the proteinaceous nuclear matrix by matrix-associated regions (MARs). Although this organization is well characterized in somatic cells, comparatively little is known of this mode of organizing the genome in the human sperm nucleus. To define this relationship, the interaction of human sperm chromatin with the nuclear matrix was assessed by fluorescence in situ hybridization using specific alpha satellite probes directed to the centromeric regions of chromosomes 13 plus 21 and 18. Hybridization of the centromeric sequences was visualized as segmented, bundled structures that extended from the nuclear core into the halo. |
| Databáze: | OpenAIRE |
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