C. elegansestablishes germline versus soma by balancing inherited histone methylation
| Autor: | Dexter A. Myrick, Teresa W. Lee, Jovan S. Brockett, David J. Katz, Caroline F. Plott, Brandon S. Carpenter |
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| Rok vydání: | 2020 |
| Předmět: |
0303 health sciences
animal structures Methyltransferase biology Somatic cell Germline Cell biology 03 medical and health sciences 0302 clinical medicine Histone Histone methylation biology.protein Demethylase Ectopic expression Reprogramming 030217 neurology & neurosurgery 030304 developmental biology |
| DOI: | 10.1101/2020.01.22.914580 |
| Popis: | Embryos undergo extensive reprogramming at fertilization to prevent the inappropriate inheritance of histone methylation. InC. elegans,this reprogramming is mediated by the H3K4me2 demethylase, SPR-5, and the H3K9 methyltransferase, MET-2. In contrast to this reprogramming, the H3K36 methyltransferase, MES-4, maintains H3K36me2/3 at germline genes between generations to help re-establish the germline. To determine whether the MES-4 germline inheritance system antagonizesspr-5; met-2reprogramming, we examined the interaction between these two systems. We find that the developmental delay ofspr-5; met-2mutant progeny is associated with ectopic H3K36me2/3 and the ectopic expression of MES-4 targeted germline genes in somatic tissues. Furthermore, the developmental delay is dependent upon MES-4 and the H3K4 methyltransferase, SET-2. We propose that the MES-4 inheritance system prevents critical germline genes from being repressed by maternalspr-5; met-2reprogramming. Thus, the balance of inherited histone modifications is necessary to distinguish germline versus soma and prevent developmental delay. |
| Databáze: | OpenAIRE |
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