High expression vectors for a Zygosaccharomyces rouxii host

Autor:	Ikuyo Utatsu, Toshiaki Imura, Akio Toh-e
Rok vydání:	1989
Předmět:	Reporter gene Expression vector biology Saccharomyces cerevisiae lac operon Promoter biology.organism_classification Molecular biology General Biochemistry Genetics and Molecular Biology Biochemistry Transcription (biology) Gene expression General Agricultural and Biological Sciences Gene
Zdroj:	Agricultural and Biological Chemistry. 53:813-819
ISSN:	1881-1280 0002-1369
Popis:	We evaluated the expression of three yeast genes in Zygosaccharomyces rouxii using lacZ as a reporter gene; they were the glyceraldehyde-3-phosphate dehydrogenase gene of Z. rouxii (GAP-Zr), that of S. cerevisiae (GAP-Sc) and the PH05 gene of S. cerevisiae. The GAP-Sc promoter showed high expression in the Z. rouxii host as well as in the S. cerevisiae host. The GA P-Zr promoter showed a comparable level of expression as the GAP-Sc both in the S. cerevisiae host and the Z. rouxii host. The transcription initiation site of the GA P-Zr promoter in the heterologous host seems to be the same as that in the homologous host. The PH05 promoter showed constitutive expression, as efficient as in the case of the GAP-Zr promoter, in the Z. rouxii host. All the promoters mentioned above can be used as sources of a high expression vector in the Z. rouxii host.
Databáze:	OpenAIRE
Externí odkaz:	https://explore.openaire.eu/search/publication?articleId=doi_________::e3553d373cbf898dc9c28965838a6d8e https://doi.org/10.1271/bbb1961.53.813 Zobrazit plný text záznamu