Simple method of open skin wound healing model in corticosteroid-treated and diabetic rats: standardization of semi-quantitative and quantitative histological assessments
| Autor: | B. Vidinsky, Petr Gál, Nikita Bobrov, Zoltán Tomori, Kilík R, Michal Mokry, Juraj Bober, L. Pasteur, L. Lenhardt, Tomáš Vasilenko, Mozes S |
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| Rok vydání: | 2008 |
| Předmět: |
Pathology
medicine.medical_specialty integumentary system General Veterinary Skin wound 040301 veterinary sciences medicine.drug_class Angiogenesis business.industry Granulation tissue 04 agricultural and veterinary sciences Surgery 0403 veterinary science 03 medical and health sciences 0302 clinical medicine medicine.anatomical_structure 030220 oncology & carcinogenesis medicine Van Gieson's stain Full thickness skin Corticosteroid Wound healing business Semi quantitative |
| Zdroj: | Veterinární medicína. 53:652-659 |
| ISSN: | 1805-9392 0375-8427 |
| Popis: | The use of a simple and reproducible model is inevitable for objective statement of the effects of external factors on wound healing. Hence, present study was conducted to establish an excisional model of skin wound healing in corticosteroid treated, and streptozotocine induced diabetic rats as well as to standardized the semi-quantitative and quantitative evaluation of selected parameters. Round full thickness skin wounds were per - formed on the back of male Sprague-Dawley rats. Animals were sacrificed two, six, and fourteen days after surgery. Sections were stained with hematoxylin-eosin and van Gieson. Both semi-quantitative (wound reepithelization; presence of: inflammatory cells, fibroblasts, new wessels, and collagen) and quantitative methods (polymorphonu- clear leucocytes/tissue macrophages ratio, percentage of re-epithelization, area of the granulation tissue) were used to evaluate the histological changes during wound healing. As compared to the control group the wound healing process of both experimental groups was decelerated. Interestingly, wound reepithelization and angiogenesis were significantly inhibited only in the steroid rats while epithelization was accelerated in diabetic rats. In conclusion, when compared to primary sutured wound healing it can be concluded that the excisional model is more appro- priate for histological assessment of the effect of various factors on wound healing. In addition, administration of corticosteroids represents simple and inexpensive model of a complex skin wound healing impairment. |
| Databáze: | OpenAIRE |
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