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This chapter focuses on the contributions of radioisotopes to weed research by following the historical development in the field. The herbicide technology, combined with the availability of the radioactivity detection technology, especially liquid scintillation counter (LSC), led to the application of radioisotopes in weed research. The domestication time of crops was usually determined by C dating, which depends on C radioactivity measurement using the LSC. A. S. Crafts invented the autoradiography technique in 1958 and it has been used in radioactive isotope research in weed science as a classic technique. Recently, V. K. Nandula and W. K. Vencill and Mendes et al. reviewed the general methods, procedure, and equipment for absorption and translocation of C-labeled herbicide in weeds excellently. The invention of the autoradiography technique to detect radioisotope translocation in plants was attributed to Crafts. For example, weed scientists used to spike C-labeled compounds on plants to trace their absorption, translocation, and metabolism and fate in the environment. |
