Mechanosensitive enteric neurons: incidence and abundance in the porcine submucosal plexus with ultrafast neuroimaging and immunhistochemical techniques v1

Autor: Gemma Mazzuoli-Weber, Kristin Elfers, Anna Katharina Filzmayer
Rok vydání: 2020
Předmět:
DOI: 10.17504/protocols.io.bpcamise
Popis: This protocol is to investigate the sensitivity of enteric neurons to mechanical stimulation in the inner submucosal plexus of the porcine colon using ultrafast neuroimaging and immunhistochemical techniques. Ultrafast neuroimaging technique combined with a voltage sensitive dye was used to characterize neuronal responses to mechanical stimuli compression and tension. Immunohistochemistry was subsequently used to determine the neurochemical coding of mechanosensitive submucosal neurons.
Databáze: OpenAIRE