Identification and expression of Pen c 2, a novel allergen from Penicillium citrinum
| Autor: | Bor-Luen Chiang, Chia-Jung Yu, Ning-Yuan Su, Lu-Ping Chow, Horng-Der Shen |
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| Rok vydání: | 1999 |
| Předmět: | |
| Zdroj: | Biochemical Journal. 341:51-59 |
| ISSN: | 1470-8728 0264-6021 |
| Popis: | The mould genus, Penicillium, is known to be a significant source of environmental aero-allergens. One important allergen from Penicillium citrinum, Pen c 2, has been identified by means of two-dimensional immunoblotting using IgE-containing patients' sera. This novel allergen was cloned, sequenced and expressed in Escherichia coli. The cloned cDNA encodes a large 457-amino acid protein precursor containing a 16-amino acid signal peptide, a 120-amino acid propeptide and the 321-amino acid mature protein. Comparison of the Pen c 2 sequence with known protein sequences revealed shared high sequence similarities with two vacuolar serine proteases from Aspergillus niger and Saccharomyces cerevisiae. Asp-46, His-78 and Ser-244 were found to constitute the catalytic triad of the 39-kDa Pen c 2. The DNA coding for Pen c 2 was cloned into vector PQE-30 and expressed in E. coli as a His-tag fusion protein that bound serum IgE from Penicillium-allergic patients on immunoblots. Recombinant Pen c 2 could therefore be used effectively for diagnosis and also potentially for the treatment of mould-derived allergic disorders. |
| Databáze: | OpenAIRE |
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