Use of mass spectrometry to compare three O-chain-linked and free lipopolysaccharide cores: differences found in Bordetella parapertussis
| Autor: | H. Zarrouk, D Karibian, Martine Caroff, I. Godard, Malcolm B. Perry |
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| Rok vydání: | 1997 |
| Předmět: |
0301 basic medicine
Salmonella ohio Lipopolysaccharide 030106 microbiology Immunology Heptose medicine.disease_cause Mass spectrometry Microbiology Bordetella parapertussis 03 medical and health sciences chemistry.chemical_compound 0302 clinical medicine Desorption medicine Molecule Molecular Biology Escherichia coli chemistry.chemical_classification Chromatography biology Cell Biology biology.organism_classification Infectious Diseases chemistry 030215 immunology |
| Zdroj: | Journal of Endotoxin Research. 4:453-458 |
| ISSN: | 0968-0519 |
| DOI: | 10.1177/096805199700400609 |
| Popis: | Plasma desorption mass spectrometry and matrix-assisted laser desorption/ionization mass spectrometry methods were used to investigate the molecular differences between lipopolysaccharide free core molecules and core molecules substituted by O-chains in Bordetella parapertussis, Salmonella ohio, and Escherichia coli 0119. The B. parapertussis analysis indicated a difference in mass of 569 amu corresponding to 3 distal sugars comprising terminal residues of heptose, galactosaminuronic acid, and, N-acetyl-N-methylfucosamine, a result supported by evidence from NMR and serology. No differences were evident in the analyses of cores in either S. ohio or E. coli O119, although the first O-chain unit carried by S. ohio core lacked a terminal glucose present in the residual O-chain repeating units. |
| Databáze: | OpenAIRE |
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