DNA protective and DNA damage activity of induced peanut hairy root crude extract with antioxidant and pro-oxidant properties

Autor: Apinun Limmongkon, Pimpimon Lumpa, Kanitha Supdensong, Vijakhana Pilaisangsuree, Jintana Pankam
Rok vydání: 2021
Předmět:
Zdroj: Acta Physiologiae Plantarum. 43
ISSN: 1861-1664
0137-5881
DOI: 10.1007/s11738-021-03326-x
Popis: The pre-exposure of methyl jasmonate (MJ) plus methyl-β-cyclodextrin (CD) followed by cadmium (Cd) extensively induced highest ferric reducing antioxidant power (FRAP) in the hairy root culture. The low concentration of crude extract exhibited DNA-protective activity. However, the pro-oxidant activity of crude extract is increased in a concentration-dependent manner. A combination of elicitors was applied to enhance secondary metabolite production in peanut hairy root culture. The increasing biosynthesis of secondary metabolites with high antioxidant capacity could be a significant mechanism in the defence response of plants to neutralise cellular oxidative stress. The sequential treatment of methyl jasmonate (MJ) plus methyl-β-cyclodextrin (CD) and cadmium (Cd), namely the (MJ + CD)_Cd strategy, exhibited the highest ferric reducing antioxidant power (FRAP) with 10.68 ± 0.17 mg ascorbic acid/g hairy root dry weight, the highest O2•−-scavenging capacity with an IC50 value of 0.89 ± 0.45 µg/mL and the highest H2O2-scavenging ability with an IC50 value of 133.90 ± 11.96 µg/mL, which showed a significant increase from the untreated control group. A potent DNA nicking assay was used to investigate the antioxidant and pro-oxidant activity of crude extracts. Low concentrations (25 µg/mL) of crude-elicited hairy root extract exhibited DNA-protective activity against free radical-mediated DNA damage, whereas higher concentrations (75 and 125 µg/mL) exhibited pro-oxidant activity in the Fenton reaction. In addition, a crude extract concentration of 50 µg/mL could directly cause DNA damage in the non-Fenton reaction. Based on the evidence that elicited bioactive compounds induced both antioxidant and pro-oxidant activity in a concentration-dependent manner, the hairy root extracts would be considered as health-beneficial compounds and can be further applied as health-promoting or cancer chemotherapeutic agents.
Databáze: OpenAIRE
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