Double-layer flexible neuronal probe with closely spaced electrodes for high-density in-vivo brain recordings
| Autor: | Francisca Machado, José Higino Correia, L. R. Jacinto, Joao Gaspar, João Ribeiro, M. J. Maciel, Sara Pimenta, José A. Rodrigues, Oleksandr Bondarchuk, Patricia Monteiro |
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| Rok vydání: | 2020 |
| Předmět: |
Fabrication
Materials science Rigidity (psychology) 02 engineering and technology Substrate (electronics) 021001 nanoscience & nanotechnology Stiffening Characterization (materials science) Metrology 03 medical and health sciences 0302 clinical medicine Electrode 0210 nano-technology Lithography 030217 neurology & neurosurgery Biomedical engineering |
| Popis: | Flexible probes for brain activity recordings are an attractive emerging approach that reduces mechanical mismatch between probe and neuronal tissue, thus minimizing the risk of brain damage or glial scaring. Although promising, flexible probes still present some technical challenges namely: i) how to overcome probe buckling during brain insertion given its intrinsically low mechanical rigidity; ii) how to fabricate closely spaced electrode configurations for high density recordings by standard lithography techniques in the flexible substrate. Here, we present a new flexible probe based solely on standard and low-cost lithography processes, which has closely spaced 10 μm diameter gold electrode sites on a polyimide substrate with inter-site distances of only 5 μm. By using a double-layer design and fabrication approach we were able to accommodate closely spaced electrode sites at two different depths from probe surface while also providing additional stiffening, just sufficient to prevent probe buckling during brain insertion. Detailed probe characterization through metrology of structural and electrical properties and chemical composition analysis, as well as functional assessment through in vivo high-density recordings of neuronal activity in the mouse cortex, confirmed the viability of this new fabrication approach and that this probe can be used for obtaining high quality brain recordings with excellent signal-to-noise ratio (SNR). |
| Databáze: | OpenAIRE |
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