EGR1 is essential for transcriptional regulation of BMPR2

Autor:	Thomas R. Blackwell, Kirk B. Lane, Radhika Gaddipati, Kirsten A. Lane, Nicole M. Lane, James West, James E. Loyd
Rok vydání:	2011
Předmět:	Genetics DNA binding site endocrine system Psychiatry and Mental health Transcription (biology) Transcriptional regulation EGR1 Repressor Promoter Transfection Biology Binding site
Zdroj:	American Journal of Molecular Biology. :131-139
ISSN:	2161-6663 2161-6620
Popis:	In this study, RLM-RACE was used to identify the transcriptional start site 387 bp upstream of the translational start. Evolutionarily conserved transcription factor binding sites were identified, and a series of luciferase reporter constructs driven by BMPR2 promoter elements used to determine their functional relevance. We found the promoter area from 983 bp to 90 bp upstream of the transcriptional start gave maximal activity, greater than longer constructs, with an area between 570 bp and 290 bp upstream of the transcriptional start containing an important repressor element. To characterize this repressor, we used a combination of EMSA, mutation of the EGR1 binding site, transfection with EGR1 and NAB1 constructs, and mutation of the NAB1 binding site within the EGR1 protein. From this we conclude that EGR1 is essential to BMPR2 transcription, but that NAB1 binding to EGR1 causes it to act as a repressor.
Databáze:	OpenAIRE
Externí odkaz:	https://explore.openaire.eu/search/publication?articleId=doi_________::e311d9d909ab0dfd28a4269e63499122 https://doi.org/10.4236/ajmb.2011.13014 Zobrazit plný text záznamu