Effect of miR-515-5p on Proliferation and Drug Sensitivity of Retinoblastoma Cells
| Autor: | Xiang Wen Yuan, Huilin Tong, Ting Qin Yan |
|---|---|
| Rok vydání: | 2020 |
| Předmět: |
0301 basic medicine
Cell growth Retinoblastoma Chemistry Cancer medicine.disease In vitro 03 medical and health sciences 030104 developmental biology 0302 clinical medicine Oncology Downregulation and upregulation Cell culture In vivo 030220 oncology & carcinogenesis microRNA Cancer research medicine |
| Zdroj: | Cancer Management and Research. 12:12087-12098 |
| ISSN: | 1179-1322 |
| DOI: | 10.2147/cmar.s271165 |
| Popis: | Background Retinoblastoma (RB) is a common malignancy in children eyes. Aberrant microRNA (miR) expression is observed in many cancer cases. miR-515-5p is reported to be concerned with the course of many cancers. This study explores the role of miR-515-5p in proliferation and drug sensitivity of RB cells. Methods Human RB cell lines (WERI-RB1, SO-RB50 and Y79) and human retinal pigment epithelial cell line ARPE-19 were utilized in this study. Drug-resistant cells SO-RB50/VCR and SO-RB50/CBP were constructed for the following experiments. The expressions of miR-515-5p and Notch1 in RB cells were detected. Notch1 was significantly upregulated in RB cells while miR-515-5p was notably downregulated. Then, the binding relationship between miR-515-5p and Notch1 was predicted and verified. Results miR-515-5p negatively regulated Notch1 expression. In vitro and in vivo experiments revealed that overexpressed miR-515-5p inhibited RB cell proliferation and enhanced drug sensitivity. Functional rescue experiment suggested that miR-515-5p regulated RB cell proliferation and drug sensitivity via inhibiting Notch1 expression. Conclusion It could be concluded that overexpressed miR-515-5p suppressed proliferation and drug resistance of RB cells by targeting Notch1 expression, indicating that miR-515-5p might constitute a promising therapy target for RB. |
| Databáze: | OpenAIRE |
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