Molecularly imprinted polymer with assistant recognition polymer chains for bovine serum albumin
| Autor: | Fan Zhi, Guo MinJie, Xia JianJun Mi HuaiFeng, Gao Ting, Yao Jing-xia |
|---|---|
| Rok vydání: | 2010 |
| Předmět: |
chemistry.chemical_classification
Acrylate biology General Chemical Engineering Molecularly imprinted polymer General Chemistry Polymer Biochemistry Polyvinyl alcohol chemistry.chemical_compound Monomer Adsorption chemistry Polymerization Materials Chemistry biology.protein Bovine serum albumin Nuclear chemistry |
| Zdroj: | SCIENTIA SINICA Chimica. 40:282-290 |
| ISSN: | 1674-7224 |
| DOI: | 10.1360/zb2010-40-3-282 |
| Popis: | A new protein molecularly imprinted polymer (MIP) was prepared with grafting polyvinyl alcohol as assistant recognition polymer chains (ARPCs). The ARPCs and acrylamide monomers were interpenetrated and then polymerized on the surface of macroporous acrylate adsorbent spheres. The template BSA was removed by treatment with 2.00 mol•L −1 potassium chloride (KCl) solution and the adsorbed proteins were detected with sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE). 0.150 mol•L −1 , 0.500 mol•L −1 , and 2.00 mol•L −1 KCl solutions were used as eluent to wash the adsorbed proteins. The SDS-PAGE results show that proteins washed out with 2.00 mol•L −1 KCl solution were from nonspecific adsorption of macroporous acrylate adsorbent spheres, and proteins washed out with 0.500 mol•L −1 KC1 solution were specific proteins imprinted by MIP resins. MIP resins with ARPCs had better recognition to the target proteins than that without ARPCs. The adsorption capacity of MIP resins immobilized ARPCs to the template BSA was about 80-100μg•g −1 when it was used for the adsorption of proteins mixture, and the specific adsorption of the target protein was obviously increased. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
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