Chemotactic Factor for Polymorphonuclear Leukocytes Derived from Human Lung Giant Cell Carcinoma Lu65 Cells

Autor:	Yoichi Taya, Toshio Fujikura, Kazuo Suzuki, Yukio Shimosato, Setsuo Hirohashi, Hiromi Ota, Atsushi Kuramoto
Rok vydání:	1989
Předmět:	Giant Cell Carcinoma Pathology medicine.medical_specialty Nutrition and Dietetics Clinical Biochemistry Size-exclusion chromatography Cell Medicine (miscellaneous) Chemotaxis Biology Molecular biology Human lung medicine.anatomical_structure Cell culture Sephadex medicine Gene
Zdroj:	Journal of Clinical Biochemistry and Nutrition. 6:147-153
ISSN:	1880-5086 0912-0009
DOI:	10.3164/jcbn.6.147
Popis:	A chemotactic factor for polymorphonuclear leukocytes was found in the culture fluid of human lung giant cell carcinoma cell line Lu65. This factor was defined as chemotactic one by checkerboard analysis. Partial purification of the chemotactic factor was performed by DEAE-Sepharose and gel filtration with Sephadex G-100, which showed it to be basic and low molecular weight. The factor was also found in the filtrate of culture fluid passed through a Centriflo CF25 filter, thus indicating a molecular weight of less than 25kDa. Furthermore, three cell clones having higher activity of chemoattractant were selected from the original cell line.
Databáze:	OpenAIRE
Externí odkaz:	https://explore.openaire.eu/search/publication?articleId=doi_________::e2da96cdc57c451579c68c4f360be675 https://doi.org/10.3164/jcbn.6.147 Zobrazit plný text záznamu