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Freshly harvested Pleurotus nebrodensis fruit bodies were exposed to four different doses (0.8–2.0 kGy) of 60 Co γ-irradiation and various physiological changes associated with postharvest deterioration, as well as the activities of selected enzymes (proteinase, polyphenoloxidase, superoxide dismutase, catalase) thought to play a role in the process of deterioration, were monitored during 22 days of subsequent storage at 4 °C and 65–70% relative humidity. An irradiation dose of 1.2 kGy significantly delayed (by 6–9 days) the onset of fruit body softening, splitting and browning compared with non-irradiated controls and test samples subjected to lower or higher irradiation doses. Irradiation with 1.2 and 1.6 kGy also had a positive effect on other indicators of mushroom tissue senescence, resulting in smaller decreases in soluble protein levels and more protracted increases in proteinase activity. Peak levels of polyphenoloxidase activity, widely recognized as causing postharvest browning of mushroom tissue, were also significantly lower ( P P. nebrodensis , and improve the prospects of more targeted strategies for extending the shelf life of both this and other mushrooms. |
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