Generalized Physicochemical Model for the Natural pH Gradient in Classic IEF

Autor:	Vanio Ivanov Mitev, Cyrill Naydenov, E. Kirazov
Rok vydání:	2014
Předmět:	Mass transport Chemistry Isoelectric focusing Diffusion Organic Chemistry Clinical Biochemistry Analytical chemistry Electrolyte Biochemistry Analytical Chemistry Matrix (mathematics) Electrokinetic phenomena Chemical physics Ph gradient Transport phenomena
Zdroj:	Chromatographia. 77:591-602
ISSN:	1612-1112 0009-5893
Popis:	In this paper we present a generalized physicochemical model for carrier ampholyte isoelectric focusing techniques. Accurate analysis of electrokinetic phenomena can only be performed when the physicochemical interactions of the carrier matrix with the electrode solutions are taken into consideration. It should also be taken into account that the majority of the constituents of electrolyte systems are weak, multivalent acids and bases, which have complex stoichiometric behavior, intricately linked with local transport phenomena. A generalized formulation to couple the migration/diffusion of weak electrolytes with transport phenomena is presented along with a broad treatment of macroscopic transport, which leads ultimately to the creation of an ampholyte pH gradient. These formulations and a novel scheme for the mechanism of mass transport constitute the core of the present paper.
Databáze:	OpenAIRE
Externí odkaz:	https://explore.openaire.eu/search/publication?articleId=doi_________::e2d27fad5c6f02b5ba741edfcbe15856 https://doi.org/10.1007/s10337-014-2644-6 Zobrazit plný text záznamu Full text from SpringerLink