| Autor: |
Shanta Dutta, Saptarshi Banerjee, Suvrotoa Mitra, Indranath Banerjee, Subhanita Roy, Mamta Chawla-Sarkar, Aratrika De, Suman Chakraborty, Nandita Kedia, Sujay Kumar Biswas, Arindam Mondal, Ritobrata Goswami, Rakesh Sarkar, Aditya Bandopadhyay |
| Rok vydání: |
2021 |
| Předmět: |
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| DOI: |
10.1101/2021.04.06.21254740 |
| Popis: |
We report a novel piece-wise isothermal nucleic acid test (PINAT) for diagnosing pathogen-associated RNA that embeds an exclusive DNA-mediated specific probing reaction with the backbone of an isothermal reverse-transcription cum amplification protocol as a unified single-step procedure. This single step sample-to-result test method has been seamlessly integrated in an inexpensive, scalable, pre-programmable and portable instrument, resulting in a generic platform technology for detecting nucleic acid from a wide variety of pathogens. The test exhibited high sensitivity and specificity of detection when assessed using 200 double-blind patient samples for detecting SARS-CoV-2 infection conducted by the Indian Council of Medical Research (ICMR), reporting a positive and negative percent agreement of 94.6% and 98% respectively. We also established its efficacy in detecting influenza-A infection, performing the diagnosis at the point of collection with uncompromised detection rigor. The envisaged trade-off between advanced laboratory-based procedures with the elegance of common rapid tests renders the innovation to be ideal for deployment in resource-limited settings towards catering the needs of the underserved. |
| Databáze: |
OpenAIRE |
| Externí odkaz: |
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