Combining different assays and chemical analysis to characterize the genotoxicity of waters impacted by textile discharges
| Autor: | Gisela de Aragão Umbuzeiro, Alain Devaux, Michel DeMeo, Josiane Aparecida de Souza Vendemiatti, Francine Inforçato Vacchi, Sylvie Bony, Vanessa Brosselin, Maria Valnice Boldrin Zanoni, Bianca Ferreira da Silva |
|---|---|
| Rok vydání: | 2016 |
| Předmět: |
0301 basic medicine
Epidemiology DNA damage Chemistry Health Toxicology and Mutagenesis fungi food and beverages Mutagen 010501 environmental sciences medicine.disease_cause 01 natural sciences 6. Clean water Upstream and downstream (DNA) Toxicology Comet assay 03 medical and health sciences 030104 developmental biology Environmental chemistry medicine Microsome Bioassay Fish Genetics (clinical) Genotoxicity 0105 earth and related environmental sciences |
| Zdroj: | Environmental and Molecular Mutagenesis. 57:559-571 |
| ISSN: | 0893-6692 |
| DOI: | 10.1002/em.22034 |
| Popis: | Waters receiving textile discharges can exhibit genotoxic and mutagenic activity, which has been related to the presence of dyes and aromatic amines as synthesis precursors or byproducts. The aim of this study was to identify dyes and aromatic amines in water samples impacted by textile discharges, and to evaluate the genotoxic responses of these samples using the Salmonella/microsome assay in strains TA98 and YG1041, and the Fpg-modified comet assay in the RTL-W1 fish cell line. The genotoxicity of river samples downstream of the discharge was greater than the upstream samples in both of the Ames tests. The Fpg-modified comet assay detected similar levels of DNA damage in the upstream and downstream samples. Mutagenicity was not detected with TA98, except for the Quilombo River samples, but when YG1041 was used as the tester strain mutagenicity was detected for all sites with a very different profile in upstream sites relative to the other sites. The mutagenic response strongly indicated that aromatic amines or dyes were contributing to the mutagenic activity downstream. The impact of textile discharges was also confirmed by chemical analysis, because the highest concentrations of azo dyes and aromatic amines were detected in the river downstream. This study shows the value of combining assays measuring complementary endpoints to better characterize the mutagenicity of environmental samples, with the advantage that this approach provides an indication of what classes of compounds are responsible for the effect. Environ. Mol. Mutagen. 57:559-571, 2016. © 2016 Wiley Periodicals, Inc. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|
Plný text