Micropropagation and clonal fidelity assessment of acclimatized plantlets of Crotalaria longipes Wight & Arn. using ISSR markers
| Autor: | Hariharan Selvam, Kaliamoorthy Seventhilingam, Balaji V. Kalaivanan |
|---|---|
| Rok vydání: | 2021 |
| Předmět: |
0106 biological sciences
0301 basic medicine fungi food and beverages Plant physiology Coco peat Plant Science Biology 01 natural sciences 03 medical and health sciences Horticulture 030104 developmental biology Micropropagation Thidiazuron Shoot Legume 010606 plant biology & botany Explant culture Crotalaria longipes |
| Zdroj: | Vegetos. 34:325-331 |
| ISSN: | 2229-4473 |
| DOI: | 10.1007/s42535-021-00203-3 |
| Popis: | An efficient protocol for micropropagation of Crotalaria longipes Wight & Arn., an endemic and endangered Indian legume, was developed. Nodal explants cultured on Murashige and Skoog (MS) supplemented with Thidiazuron (TDZ) 1.0 mg L−1 and NAA 0.5 mg L−1 showed maximum shoot induction. The multiplied shoots were subsequently sub-cultured every 3 weeks on the same medium. The separated microshoots cultured on half-strength MS basal salts supplemented with 0.5 mg L−1 indole-3-butyric acid (IBA) showed maximum root induction. After 4 weeks of culture, the rooted shoots (> 6 cm) were planted in pots containing coco peat and garden soil (1:1) covered with plastic domes and maintained at 25 oC for 2 weeks. After 2 weeks, the potted plants were transferred to a glasshouse, maintained at 25 °C with a relative humidity of 80%. The acclimatized plants were then successfully established in soil with an 80% survival rate. Clonal fidelity assessment of acclimatized plantlets was confirmed using inter simple sequence repeat (ISSR). All the in vitro developed plants showed monomorphic banding pattern similar to the mother plant, thus ascertaining the true-to-type nature of the in vitro raised plants. |
| Databáze: | OpenAIRE |
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