Reconstitution of the Steroid Receptor Heterocomplex
| Autor: | Gisela Ileana Mazaira, Mario D. Galigniana |
|---|---|
| Rok vydání: | 2019 |
| Předmět: |
0301 basic medicine
biology Chemistry medicine.medical_treatment Hsp90 Cell biology 03 medical and health sciences Steroid hormone 030104 developmental biology 0302 clinical medicine Glucocorticoid receptor Nuclear receptor 030220 oncology & carcinogenesis Chaperone (protein) biology.protein medicine Signal transduction Receptor Transcription factor |
| Zdroj: | Methods in Molecular Biology ISBN: 9781493991945 |
| DOI: | 10.1007/978-1-4939-9195-2_10 |
| Popis: | Steroid receptors are members of a subfamily of the nuclear receptor superfamily. They play a dual role of steroid hormone receptors and transcription factors. Actually, these receptors are steroid-activated transcription factors. Classical soluble receptors exist as oligomeric complexes with the Hsp90-based chaperone machinery. The steroid receptor field was born and developed along with the molecular chaperone field. Chaperones are not exclusive partners associated to these receptors, but also comprise a large variety of heterocomplexes with other proteins involved in signal transduction. By using the glucocorticoid receptor (GR) as a standard model for most Hsp90-client proteins, in this chapter we describe the functional GR·Hsp90 heterocomplex assembly system from reticulocyte lysate or purified proteins. |
| Databáze: | OpenAIRE |
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