Nucleolus Extrusion: An Electron-microscopic Study of Human Esophageal Cancer Cell Lines under Hypothermia

Autor:	Masaru Sawataishi, Tomohiro Saitoh, Mitsukazu Saitoh, Katsunori Tauchi, Masao Fujimaki, Toshiko Yoshida, Takashi Shinbo, Yoshiaki Karaki, Kenichi Tazawa, Michio Kimura, Katsuhiro Tsukada, Kenichi Takaya, Yoshinobu Yokoyama, Takuya Nagata
Rok vydání:	2002
Předmět:	Nucleolus Cell Biology Cell biology Staining law.invention medicine.anatomical_structure Cytoplasm law medicine Ultrastructure Granular component Electron microscope Nucleus
Zdroj:	Nihon Kikan Shokudoka Gakkai Kaiho. 53:417-429
ISSN:	1880-6848 0029-0645
DOI:	10.2468/jbes.53.417
Popis:	Neoplastic cells from human esophageal cancer cell lines, SGF-3, SGF-4 and SGF-5, maintained in a hypothermic state at 21.5°C were examined under electron microscope. Ultrastructural alterations, including nucleolar lesions, were found in the cells. SGF cell nucleoli showed characteristic changes under hypothermia, consisting of a segregation of the granular component, the fibrillar center and the fibrillar component. Both morphological apoptotic features and regenerative features were disclosed by electron microscopy. Segregated nucleoli extruded from the nucleus into the cytoplasm were revealed in these hypothermic cells. RNA staining distinguished the nucleoli from the nuclear material extruded into the cytoplasm, indicating the nucleoli in the nucleus, the migration of the nucleoli into the cytoplasm and a RNA-positive material, probably nucleolus origin of budding with the cytoplasmic membrane under hypothermia.
Databáze:	OpenAIRE
Externí odkaz:	https://explore.openaire.eu/search/publication?articleId=doi_________::e269356b70fe6a5b2529be290268f85d https://doi.org/10.2468/jbes.53.417 Zobrazit plný text záznamu