Oligonucleotide fingerprinting of tomato DNA

Autor:	Thomas Börner, D. Kaemmer, Jörg T. Epplen, B. Beyermann, G. Kahlm, Kurt Weising
Rok vydání:	1995
Předmět:	Genetics biology Oligonucleotide Dendrogram Plant Science biology.organism_classification chemistry.chemical_compound DNA profiling chemistry Polymorphism (computer science) Microsatellite Cultivar Agronomy and Crop Science Solanaceae DNA
Zdroj:	Plant Breeding. 114:12-17
ISSN:	1439-0523 0179-9541
DOI:	10.1111/j.1439-0523.1995.tb00751.x
Popis:	HinfI-digested DNA samples of wild and cultivated species of tomato were hybridized in get to different oligonucleotide probes complementary to simple-sequence repeats. All motifs tested i.e. (GATA) 4 , (GGAT) 4 , (CA) 8 , (CAG) 5 , (GTG) 5 , (GAAGTGGG) 2 and (CCTA) 4 , produced multilocus fingerprints in all tomato species and cultivars. The extent of inter- and intraspecific polymorphism of the banding patterns varied considerably between different probes, with (GGAT) 4 , (CCTA) 4 , (GATA) 4 and (CA) 5 showing a much higher discriminatory potential than the other probes. Cluster analysis of band-sharing data yielded dendrograms which were consistent with the known history of tomato cultivation. (GATA) 4 was also successfully used for purity testing of breeding lines, as well as for analysing an F 1 progeny of a cross between two lines.
Databáze:	OpenAIRE
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