Increasing the Sensitivity of the 15N Root Bioassay Technique: Suggested Procedures

Autor: Helen E. Jones, Ulrika Rosengren, Darren Sleep, Gunnar Thelin
Rok vydání: 2003
Předmět:
Zdroj: Communications in Soil Science and Plant Analysis. 34:2363-2373
ISSN: 1532-2416
0010-3624
Popis: The diagnosis of tree nutrient demand for nitrogen (N), phosphorus (P), and potassium (K) is possible using a root bioassay with excised roots, to measure the rate of uptake of the corresponding isotope. In this study, Norway spruce (Picea abies) roots were used to test the uptake response in roots of different diameter. The 15 N bioassay has been developed to test for nitrogen demand by plants, where high 15 N uptake demonstrates nitrogen limitation. Sometimes sample variability may mask potential treatment differences, but we have shown that when the fine root fraction of
Databáze: OpenAIRE