Morphometric analysis of the efficiency of bone defect substitution with fabric equivalents of bone tissue on the basis of multipotent mesenchymal stromal cells in dentistry

Autor: S. V. Tkachyk, Y. V. Goritsky, A. V. Bambuliak, O. Y. Gagen
Rok vydání: 2020
Předmět:
Zdroj: Bukovinian Medical Herald. 24:18-27
ISSN: 2413-0737
1684-7903
Popis: Bone tissue is a rather dynamic tissue that has the ability to recover from injury without scarring. The differentiation of multipotent mesenchymal stromal cells (MМSC) into osteoblasts plays a crucial role in bone regeneration and remodeling. MМSC derived from adipose tissue are considered to be an adequate source for bone tissue engineering because of their capacity for osteogenic differentiation. The effectiveness of osteogenic differentiation of different human MMSC has been demonstrated in the settlement of biocompatible polymer matrices. It was found that MMSC derived from adipose tissue showed a high efficiency of differentiation into osteoblasts. These cells are typically transplanted into three-dimensional porous scaffolds that provide the necessary extracellular environment that contains physical and chemical signals for tissue development and regeneration.The aim of the study: to analyze the results of morphometric studies to determine the effectiveness of the use of tissue equivalents of bone tissue based on multipotent mesenchymal stromal fat cells in dental practice.Material and methods. The experiment was conducted on the Wistar line rats, weighing 200–250 grams, which were divided into VI groups. A bone defect model was formed in the parietal section of the skull of rats. The formed defect implanted the harvested material. Morphometric studies were performed in accordance with the principles of systematic quantitative analysis (Avtandilov GG, 2002). To do this, we used the automatic image processing system „Videotest-Morpho 3.0” (Russia), which contains a binocular research microscope „Biolam”, an analog-to-digital converter, a computer with a high-resolution color printer and software. In the regenerate, the volume particles (Vp, μm3/μm3) of connective tissue, vessels and bone beams, as well as the numerical density of cellular elements (1/μm3) were determined using morphometric methods. The obtained results are processed statistically.Results. After 3 months of observations, clear quantitative positive changes in the structure of the regenerates were observed in the skull defect area of the experimental animals. Thus, in animals of groups IV and VI the volume fraction of bone beams in regenerates increased to 43,90 ± 1,68%, p1 — p2 0.05. On the 90th day of observation there was a significant decrease in the volume fraction of vessels, which in the IV and VI groups with values 7,44 ± 0.82% and 6,15 ± 0,90%, p2 0,05. In animals of group VI, where the combination of MMSC-AT +PRP + Kolapan was used to repair the bone defect, the maximum increase was determined: the number of osteoblasts and osteocytes, the number of osteoclasts against the background of reduction of fibroblasts and fibrocytes. The normalization of cellular element cell density in group IV rats, where bone defect replacement occurred due to the combination of MMSC-AT +PRP, was quite significant. At the same time, in the animals of this group the number of osteoclasts, р1 0.05, with 1,2 times smaller number of osteoblasts and osteocytes, р>0,05 р1 — р2>0,01.Conclusions. Therefore, by the results of morphometric studies on the model defects of the skull bone of rats proved the suitability of the investigated implants for use, especially with the combination of MMSC-AT +PRP and MMSC-AT +PRP + Kolapan, which provided complete closure of the defect in 90 days.
Databáze: OpenAIRE
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