Cisplatin, Transplatin, and Their Hydrated Complexes: Separation and Identification Using Porous Graphitic Carbon and Electrospray Ionization Mass Spectrometry

Autor:	Hans Ehrsson, Anita Andersson, Inger Wallin, Per Olof G. Edlund
Rok vydání:	1995
Předmět:	chemistry.chemical_compound Electrospray chemistry Electrospray ionization Mass spectrum Aqueous two-phase system Analytical chemistry Mass spectrometry Acetonitrile Cis–trans isomerism Analytical Chemistry Nuclear chemistry Adduct
Zdroj:	Analytical Chemistry. 67:3608-3611
ISSN:	1520-6882 0003-2700
DOI:	10.1021/ac00115a035
Popis:	A new liquid chromatographic technique for the separation of the anticancer drug cisplatin, its trans isomer, and their corresponding cytotoxic hydrated complexes is presented. The separation is performed on porous graphitic carbon using an alkaline aqueous phase, where the hydrated complexes are present in their less reactive, uncharged form. The electrospray mass spectrum of cisplatin differed from that of transplatin, giving a sodiated adduct ion. The monohydrated complex of cisplatin showed [M - H 2 O] + , M + , and [M - H 2 O + MeCN] + clusters starting at m/z 263, 281, and 304, respectively, using a solvent mixture containing acetonitrile. The identity of m/z 304 was established by MS/MS.
Databáze:	OpenAIRE
Externí odkaz:	https://explore.openaire.eu/search/publication?articleId=doi_________::e2489acc06876fd9ccd490bb310f192f https://doi.org/10.1021/ac00115a035 Zobrazit plný text záznamu