| Popis: |
Cancer stem cells (CSCs) have the potential to be chemo-resistant and recurrent and/or to become metastatic tumors. It is highly critical to control the proliferation of CSCs using alternative therapies. Towards this end, we studied the proliferation control of cancer-stem-cell like mesenchymal cells using electrical pulses and Bleomycin, a FDA approved (in the US, and the respective medical agencies in the other countries) chemotherapeutic drug. For this purpose, adult human mesenchymal stem cells (hMSCs), derived from bone marrow, were used. Both low intensity, long duration (200 V/cm, 10 ms) and high intensity, short duration (1200 V/cm, 100 μs) pulses were applied and their efficacies studied. The results indicate that electrical pulses-based therapy could be utilized economically and efficiently to control the proliferation of hMSCs and shows promise of an easy transition to use in the clinic. |
