| Autor: |
Min Chen, Zhengjiang Zha, Jun Mao, Heping Zhou, Zhihong Li |
| Rok vydání: |
2021 |
| Předmět: |
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| Zdroj: |
Journal of Biomaterials and Tissue Engineering. 11:1955-1960 |
| ISSN: |
2157-9083 |
| DOI: |
10.1166/jbt.2021.2771 |
| Popis: |
Clarification of the miR-56a-mediated effect of Wnt/β-catenin pathway in glioma cells on radiosensitization. miRNA arrays were used to analyze the differential expression of miRNAs in biopsies from glioma patients. qRT-PCR to detect the levels of miR-56a and Wnt/β-catenin expressed in glioma cells and tissues. Evaluation of the impact of miR-56a on cell growth, invasion, and migrationforming ability by MTT assay and colony formation experiments. To analyze the involvement of miR-56a-mediated Wnt/β-catenin pathway in glioma biological processes and to examine the impact of miR-56a in glioma cell radiosensitivity. After miRNA array analysis, we found that miR-56a expression was significantly increased, and further studies showed that ectopic miR-56a expression in glial cells was sensitive to radiotherapy. miR-56a induction of Wnt/β-catenin promotes the upregulation of Parp in glioma cells. miR-56a can promote glioma cell migration and invasion in vitro as an important potential target for glioma disease. |
| Databáze: |
OpenAIRE |
| Externí odkaz: |
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