Sphingopyxis lindanitolerans sp. nov. strain WS5A3pT enriched from a pesticide disposal site
| Autor: | Witold Danikiewicz, Andrzej Dziembowski, Grzegorz Spólnik, Michal A. Kaminski, Leszek Lipinski, Adam Sobczak |
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| Rok vydání: | 2018 |
| Předmět: |
0301 basic medicine
Whole genome sequencing Phylogenetic tree biology Strain (chemistry) 030106 microbiology General Medicine biology.organism_classification 16S ribosomal RNA Microbiology Sphingopyxis 03 medical and health sciences Food science Sphingopyxis granuli Ecology Evolution Behavior and Systematics Bacteria Mesophile |
| Zdroj: | International Journal of Systematic and Evolutionary Microbiology. 68:3935-3941 |
| ISSN: | 1466-5034 1466-5026 |
| DOI: | 10.1099/ijsem.0.003094 |
| Popis: | An aerobic, Gram-stain-negative, rod-shaped, non-motile, mesophilic soil bacterium, strain WS5A3pT, was isolated from a pesticide burial site in north-west Poland. The strain grew at 12–37 °C, at pH 8–9 and with 0–2 % (w/v) NaCl. The main fatty acids detected in WS5A3pT were summed feature 3, summed feature 8 and C16 : 0. The major respiratory quinone was Q-10 and major polar lipids were phosphatidylethanolamine, sphingoglycolipid and phosphatidylglycerol. The G+C content of the genome was 65.1 mol%. Phylogenetic pairwise distance analysis of the 16S rRNA gene placed this strain within the genus Sphingopyxis , with the highest similarity to Sphingopyxis witflariensis W-50T (98.8 %), Sphingopyxis bauzanensis BZ30T and Sphingopyxis ginsengisoli Gsoil 250T (98.3 %) and Sphingopyxis granuli NBRC 100800T (98.09 %). Genomic similarity analyses using ANIb and dDDH algorithms indicated levels of similarity of 81.44, 80.84 and 81.16 % between WS5A3pT and S. witflariensis , S. bauzanensis and S. granuli , respectively for average nucleotide identity and 25.90, 25.00 and 26.10 % for digital DNA–DNA hybridization. Based on the phylogenetic and phenotypic data, strain WS5A3pT should be considered as a representative of a novel Sphingopyxis species. The name Sphingopyxis lindanitolerans sp. nov. is proposed with the type strain WS5A3pT (=DSM 106274T=PCM 2932T). |
| Databáze: | OpenAIRE |
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