Effects of Kumaizasa (Sasa senanensis) Leaf Extract on Innate Immune Regulation in HEK293 Cells and Macrophages

Autor:	Takaki Nedachi, Ken-Ichi Komatsu, Hiroyuki Inagawa, Akifumi Nakata, Ryosuke Tatsunami, Koji Wakame, Shinji Harakawa, Kaoru Haketa, Keisuke Sato
Rok vydání:	2021
Předmět:	MAPK/ERK pathway Cancer Research Messenger RNA Innate immune system Oncology Cell culture Chemistry Immunity Sasa senanensis HEK 293 cells General Medicine Signal transduction Cell biology
Zdroj:	Anticancer Research. 41:4093-4100
ISSN:	1791-7530 0250-7005
DOI:	10.21873/anticanres.15212
Popis:	Background/aim We investigated the effect of Kumaizasa leaf extract (KLE) on innate immunity using the HEK293 and RAW 264.7 cell lines. Materials and methods KLE, lipopolysaccharides (LPS), or KLE with LPS were added to RAW 264.7 cells. The TNF-α and IL-1β mRNA expression was then quantified. The expression of MAPKs, NFĸB, TNF-α and IL-1β proteins was also quantified. In addition, KLE was added to HEK293 cells and the IL-8 concentration was measured. Results In RAW 264.7 cells, KLE increased the levels of TNF-α and IL-1β mRNA. By contrast, when KLE and LPS were added to RAW 264.7 cells, the increase in TNF-α and IL-1β mRNA was ameliorated. Similarly, the expression of JNK and ERK proteins was reduced. The addition of KLE to HEK293 cells induced IL-8 production. Conclusion Based on these results, a KLE-mediated mechanism may regulate immunity by suppressing the expression of JNK and ERK, which are involved in inflammatory signal transduction.
Databáze:	OpenAIRE
Externí odkaz:	https://explore.openaire.eu/search/publication?articleId=doi_________::e2278c05afe6a5de5018d7ccf470a111 https://doi.org/10.21873/anticanres.15212 Zobrazit plný text záznamu