| Autor: |
R. S. Murray, Gary F. Cabirac, B. Hogue, P. J. Didier, D. M. Skolnick, K. Dorovini-Zis, L. B. McLaughlin |
| Rok vydání: |
1995 |
| Předmět: |
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| Zdroj: |
Advances in Experimental Medicine and Biology ISBN: 9781461357759 |
| DOI: |
10.1007/978-1-4615-1899-0_11 |
| Popis: |
Primary human and primate brain microvascular endothelial cells were tested for permissiveness to coronaviruses JHM and 229E. While sub-genomic viral RNAs could be detected up to 72 hours post-infection, primate cells were abortively infected and neither virus caused cytopathology. Human cells were non-permissive for JHM but permissive for 229E replication; peak production of progeny 229E and observable cytopathic effects occurred approximately 22 and 32 hour post-infection, respectively. Using the criterion of cytopathology induction in infected endothelial cells, 229E was compared to other human RNA and DNA viruses. In addition, virus induced modulation of intercellular adhesion molecule 1 (ICAM-1), vascular cell adhesion molecule 1 (VCAM-1) and HLA I was monitored by immunostaining of infected cells. |
| Databáze: |
OpenAIRE |
| Externí odkaz: |
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