Protection of conjugated linoleic acids from ruminal hydrogenation and their incorporation into milk fat

Autor: T. W. Scott, Soressa M. Kitessa, E. Fleck, J. R. Ashes, S.K. Gulati, E.B. Byers, Y.G. Byers
Rok vydání: 2000
Předmět:
Zdroj: Animal Feed Science and Technology. 86:139-148
ISSN: 0377-8401
Popis: In vitro incubations were used to assess the hydrogenation of conjugated linoleic acid (CLA) isomers 9-cis 11-trans and 10-trans 12-cis present in synthetically produced CLA-60. About 80‐ 90% of the unprotected CLA was hydrogenated when incubated at 388C for 24 h anaerobically with sheep rumen fluid, the main end product of hydrogenation being trans-octadecaenoic acid (C18:1). Encapsulation of the CLA in a matrix of protein provided a protection of about 70% with a 30% hydrogenation of the CLA isomers, resulting in no significant change in the trans-C18:1 but an increase in the level of stearic acid (C18:0). Feeding sheep with unprotected CLA or protected CLA increased the proportion of isomers 9-cis 11-trans and 10-trans 12-cis in abomasal digesta. The concentration of the CLA isomers leaving the abomasum and available for absorption at the small intestine was about 3.5‐4% higher for the protected CLA, confirming protection imparted by encapsulation. Feeding lactating goats with protected CLA increased the proportion of isomers 9-cis 11-trans and 10-trans 12-cis in milk fat. The total CLA levels were enhanced by about 10-fold above the control levels present in milk fat with an efficiency of transfer into milk fat of 36‐41% and 21‐30%, respectively, for the two isomers. # 2000 Published by Elsevier Science B.V.
Databáze: OpenAIRE
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