Flow Injection Immunosensor for Theophylline
| Autor: | Nichola J. Seare, Tony E. Edmonds, Derek A. Palmer |
|---|---|
| Rok vydání: | 1993 |
| Předmět: |
chemistry.chemical_classification
Flow injection analysis Chromatography medicine.diagnostic_test biology Elution Biochemistry (medical) Clinical Biochemistry Phosphatase Phosphate Biochemistry Analytical Chemistry chemistry.chemical_compound Enzyme chemistry Immunoassay Electrochemistry medicine biology.protein Theophylline Protein A Spectroscopy medicine.drug |
| Zdroj: | Analytical Letters. 26:1425-1439 |
| ISSN: | 1532-236X 0003-2719 |
| DOI: | 10.1080/00032719308017423 |
| Popis: | A heterogeneous on line electrochemical enzyme immunoassay has been developed for the antiasthmatic drug theophylline using a flow injection analysis system containing a protein A immunoreactor to separate antibody bound and unbound theophylline-alkaline phosphatase. In this assay the antibody, label and sample are sequentially immobilised onto the protein A immunoreactor. The antibody-protein A and antibody-protein A label/sample reaction takes place at near neutral pH, and the complexes are eluted at acid pH. p-Aminophenyl phosphate (PAPP) and p-aminophenol (PAP) have been used as the detection system. Experimental variations such as flow rate and reactant concentration have been studied and serum theophylline has been determined by this method and good r.s.d values and % recoveries have been achieved. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|